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N, N-Bis (2-hydroxyethyl)-2-aminoethanesulfonic acid sodium ntsev CAS: 66992-27-6
N, N-Bis (2-hydroxyethyl)-2-aminoethanesulfonic acid sodium ntsev, tseem hu ua HEPES sodium ntsev, yog ib qho tshuaj lom neeg feem ntau siv los ua pH buffering tus neeg sawv cev hauv cov chaw sim tshuaj lom neeg thiab tshuaj lom neeg.Nws pab tswj kom muaj pH ruaj khov, ua rau nws zoo tagnrho rau cov ntawv thov xws li kab lis kev cai ntawm tes, kev tshuaj ntsuam enzyme, kev tshawb fawb protein, electrophoresis, thiab tshuaj formulations.HEPES sodium ntsev ua kom muaj kev pom zoo rau cov txheej txheem lom neeg thiab txhim kho qhov tseeb thiab kev ntseeg siab ntawm cov txiaj ntsig kev sim.
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S-Butyrylthiocholine iodide CAS: 1866-16-6
S-Butyrylthiocholine iodide yog ib qho tshuaj sib xyaw uas feem ntau siv hauv biochemical thiab enzymatic assays.Nws yog ib qho substrate rau enzyme butyrylcholinesterase (BChE) thiab siv los ntsuas nws cov haujlwm.
Thaum S-Butyrylthiocholine iodide yog hydrolyzed los ntawm BChE, nws tsim thiocholine thiab butyric acid ua khoom.Kev tso tawm ntawm thiocholine tuaj yeem ntsuas tau siv spectrophotometric lossis fluorometric assay, tso cai kom muaj nuj nqis ntawm BChE cov haujlwm.
S-Butyrylthiocholine iodide feem ntau yog siv rau hauv chaw kho mob thiab kev tshawb fawb los soj ntsuam cov haujlwm ntawm BChE hauv cov qauv xws li ntshav ntshav lossis cov ntaub so ntswg.Nws tuaj yeem siv los ntsuas qhov ua haujlwm enzymatic ntawm BChE thiab nws lub luag haujlwm muaj peev xwm hauv ntau yam txheej txheem lom neeg, nrog rau kev kuaj mob thiab saib xyuas qee yam mob.
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ABTS (2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium ntsev) CAS: 30931-67-0
Diammonium 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate), feem ntau hu ua ABTS, yog ib qho uas nquag siv chromogenic substrate hauv biochemical ntsuam xyuas, tshwj xeeb tshaj yog nyob rau hauv lub teb ntawm enzymology.Nws yog cov khoom siv hluavtaws uas siv los ntsuas kev ua haujlwm ntawm ntau yam enzymes, suav nrog peroxidases thiab oxidases.
ABTS tsis muaj xim nyob rau hauv nws daim ntawv oxidized tab sis tig xiav-ntsuab thaum oxidized los ntawm ib qho enzyme nyob rau hauv muaj hydrogen peroxide los yog molecular oxygen.Qhov kev hloov xim no yog vim qhov tsim ntawm radical cation, uas absorbs lub teeb nyob rau hauv lub spectrum pom.
Cov tshuaj tiv thaiv ntawm ABTS thiab cov enzyme tsim cov khoom xim uas tuaj yeem ntsuas spectrophotometrically.Qhov kev siv ntawm cov xim yog ncaj qha proportional rau lub enzymatic kev ua si, tso cai rau cov kws tshawb fawb los soj ntsuam ntau npaum li cas enzyme kinetics, enzyme inhibition, los yog enzyme-substrate kev sib cuam tshuam.
ABTS muaj ntau yam kev siv hauv ntau yam, suav nrog kev kuaj mob, kev tshawb fawb tshuaj, thiab kev tshawb fawb txog zaub mov.Nws yog ib qho rhiab heev thiab muaj ntau yam dynamic ntau yam, ua rau nws yog ib qho kev xaiv nrov rau ntau qhov kev ntsuam xyuas biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS TSIS TAU: 74173-30-1
4-Nitrophenyl α-D-maltohexaoside yog ib qho chaw sib xyaw ua ke hauv chav kawm ntawm α-glycosidic linkages.Nws yog ib tug derivative ntawm maltose, uas yog ib tug disaccharide muaj li ntawm ob lub qab zib units.Hauv qhov sib xyaw no, pawg hydroxyl ntawm thawj chav tsev piam thaj yog hloov nrog nitrophenyl moiety.
Cov tshuaj no feem ntau yog siv los ua ib qho substrate hauv kev soj ntsuam enzymatic los kawm txog kev ua haujlwm ntawm ntau yam enzymes, tshwj xeeb tshaj yog cov koom nrog hauv carbohydrate metabolism.Cov pab pawg nitrophenyl tso cai rau kev tshawb nrhiav tau yooj yim thiab kom muaj nuj nqis ntawm cov tshuaj tiv thaiv enzymatic los ntawm kev ntsuas qhov nqus los yog fluorescence ntawm cov khoom cleaved.
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PIPES CAS: 5625-37-6 Chaw tsim tshuaj paus Nqe
PIPES (piperazine-1,4-bisethanesulfonic acid) yog ib qho zwitterionic buffering compound feem ntau siv hauv kev tshawb fawb lom thiab biochemical.Nws yog ib qho pH tsis zoo nrog lub peev xwm siab rau kev tswj xyuas pH ruaj khov hauv pH ntau ntawm 6.1 txog 7.5.PIPES muaj kev cuam tshuam tsawg heev rau biomolecules thiab tsim nyog rau kev ntsuas kub ntawm qhov ntsuas kub.Nws yog feem ntau siv nyob rau hauv gel electrophoresis cov tswv yim thiab tshuaj formulation ua ib tug stabilizing neeg sawv cev.Zuag qhia tag nrho, PIPES yog ntau yam thiab siv dav hauv ntau qhov chaw sim.
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3,3′,5,5′-Tetramethylbenzidine CAS: 207738-08-7
3,3′,5,5′-Tetramethylbenzidine, tseem hu ua TMB, yog ib tug tshuaj compound feem ntau siv raws li ib tug chromogenic substrate nyob rau hauv enzyme-linked immunosorbent assays (ELISA) thiab lwm yam biochemical kev soj ntsuam.Nws feem ntau siv los kuaj xyuas thiab ntsuas qhov muaj cov enzymes xws li horseradish peroxidase (HRP) hauv ntau cov qauv lom.TMB tau hloov xim ntawm cov xim tsis muaj xim mus rau xiav thaum muaj cov enzymes no.Tom qab ntawd, cov tshuaj tiv thaiv tuaj yeem nres los ntawm kev ntxiv cov kua qaub uas hloov cov xim xiav mus rau xim daj kawg.Qhov kev siv ntawm cov xim daj yog proportional rau tus nqi ntawm enzyme tam sim no, tso cai rau kom muaj nuj nqis.
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APS-5 CAS: 193884-53-6 Chaw tsim tshuaj paus Nqe
(4-Chlorophenyl) thio-methanol 1-(dihydrogen phosphate) disodium ntsev (1: 2) yog ib qho tshuaj sib xyaw uas muaj nyob rau hauv chav kawm ntawm acridine derivatives.Nws muaj 10-methylacridine nplhaib system nrog ib pawg thioether txuas rau 4-chlorophenyl txoj hauj lwm.Lub compound kuj muaj ib pab pawg methanol thiab ob pawg phosphate uas yog ib nrab neutralized los ntawm sodium ions.
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5-Bromo-4-chloro-3-indolyl phosphate disodium ntsev CAS: 102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium ntsev (BCIP) yog ib qho tshuaj lom neeg feem ntau siv hauv molecular biology thiab biochemistry daim ntaub ntawv.Nws yog chromogenic substrate rau alkaline phosphatase enzymes.
BCIP feem ntau yog siv ua ke nrog nitroblue tetrazolium (NBT) ua lub substrate los xyuas txog alkaline phosphatase kev ua.Thaum BCIP yog dephosphorylated los ntawm alkaline phosphatase, cov ntaub ntawv xiav precipitate, tso cai rau kev pom ntawm cov enzyme qhov muaj lossis kev ua haujlwm.
Cov tshuaj no tshwj xeeb tshaj yog muaj txiaj ntsig zoo hauv kev siv xws li immunohistochemistry, hauv situ hybridization, thiab enzyme-linked immunosorbent assays (ELISAs) txhawm rau txheeb xyuas qhov muaj lossis hauv cheeb tsam ntawm cov biomolecules tshwj xeeb lossis nucleic acids.Lub xiav precipitate tsim los ntawm BCIP muab lub teeb pom kev pom uas pab hauv kev txheeb xyuas thiab tshuaj xyuas ntawm lub hom phiaj molecules hauv cov qauv sim.
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Sodium ntsev ntsev CAS: 139-41-3 Chaw tsim tshuaj paus Nqe
N, N-Bis (2-hydroxyethyl) glycine sodium ntsev yog tshuaj lom neeg siv los ua tus neeg sawv cev buffering nyob rau hauv ntau yam kev siv biochemical thiab biophysical.Nws pab tswj kom muaj qib pH ruaj khov hauv kev sim, ua kom muaj txiaj ntsig zoo hauv kev tshawb fawb enzyme, kev tshawb fawb protein, kab lis kev cai ntawm tes, thiab Western blotting cov tswv yim.
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4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
4-Aminophthalhydrazide, tseem hu ua 4-APhH, yog cov tshuaj sib xyaw nrog cov mis molecular C8H8N2O.Nws belongs rau chav kawm ntawm hydrazide tebchaw thiab yog muab los ntawm phthalic acid.
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N-Acetyl-L-cysteine CAS: 616-91-1
N-Acetyl-L-cysteine (NAC) yog ib daim ntawv hloov kho ntawm cov amino acid cysteine.Nws muab ib qho chaw ntawm cysteine thiab tuaj yeem hloov mus rau hauv tripeptide glutathione, muaj zog antioxidant hauv lub cev.NAC paub txog nws cov khoom antioxidant thiab mucolytic, ua rau nws muaj txiaj ntsig zoo hauv ntau daim ntawv thov kev noj qab haus huv.
Raws li ib qho antioxidant, NAC pab tiv thaiv cov hlwb los ntawm kev puas tsuaj los ntawm cov dawb radicals, reactive oxygen hom, thiab co toxins.Nws kuj txhawb nqa glutathione synthesis, uas ua lub luag haujlwm tseem ceeb hauv lub cev cov txheej txheem detoxification thiab tswj lub cev tsis muaj zog.
NAC tau kawm txog nws cov txiaj ntsig zoo hauv kev ua pa, tshwj xeeb tshaj yog rau cov tib neeg uas muaj mob xws li mob ntsws ntev, COPD, thiab cystic fibrosis.Nws yog feem ntau siv los ua ib qho expectorant los pab nyias thiab loosen cov hnoos qeev, ua kom yooj yim rau tshem tawm cov pa.
Tsis tas li ntawd, NAC tau pom cov lus cog tseg hauv kev txhawb nqa lub siab kev noj qab haus huv los ntawm kev pab tshem tawm cov tshuaj lom, xws li acetaminophen, ib qho tshuaj tua kab mob.Nws kuj tseem muaj peev xwm tiv thaiv lub siab puas tsuaj los ntawm kev haus cawv.
Ntxiv nrog rau nws cov tshuaj tiv thaiv antioxidant thiab kev ua pa, NAC tau tshawb fawb txog nws cov txiaj ntsig zoo hauv kev puas siab puas ntsws.Qee qhov kev tshawb fawb qhia tias nws yuav muaj kev cuam tshuam zoo rau kev xav tsis zoo, xws li kev nyuaj siab thiab obsessive-compulsive disorder (OCD).
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Acetylthiocholine iodide CAS 1866-15-5
Acetylthiocholine iodide yog cov tshuaj sib xyaw uas feem ntau siv los ua cov txheej txheem hauv cov tshuaj enzyme los ntsuas kev ua haujlwm ntawm enzyme acetylcholinesterase (AChE).AChE yog ib qho enzyme uas hydrolyzes neurotransmitter acetylcholine, ib kauj ruam tseem ceeb hauv kev txiav cov teeb liab kis ntawm cov paj hlwb.
Thaum acetylthiocholine iodide ua raws li AChE, pawg acetyl raug tshem tawm, uas ua rau tsim cov thiocholine thiab acetate ions.Thiocholine ces reacts nrog cov xim tsis muaj xim hu ua DTNB (5,5'-dithiobis (2-nitrobenzoic acid)) los tsim cov xim daj xim hu ua 5-thio-2-nitrobenzoate, uas tuaj yeem ntsuas spectrophotometrically.Tus nqi ntawm kev loj hlob xim yog ncaj qha proportional rau cov dej num ntawm AChE hauv cov qauv.
