5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS: 4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) yog ib qho chromogenic substrate feem ntau siv rau kev tshawb nrhiav beta-glucuronidase (GUS).GUS yog ib qho enzyme uas muaj nyob rau hauv ntau yam kab mob, nrog rau cov kab mob, nroj tsuag, thiab tsiaj txhu.X-Gluc feem ntau siv hauv GUS tus kws tshuaj ntsuam xyuas thiab kev tshawb fawb molecular biology.

Daim ntawv thov tseem ceeb ntawm X-Gluc yog nyob rau hauv cov txheej txheem histochemical staining, qhov twg nws tuaj yeem pom qhov kev qhia thiab kev ua haujlwm ntawm GUS enzyme.Cov substrate no yog cell-permeable thiab dhau los ua hydrolyzed los ntawm GUS, uas ua rau tsim ib qho xiav precipitate los yog insoluble khoom.Qhov xim xiav no tso cai rau cov kws tshawb fawb los txheeb xyuas thiab txheeb xyuas cov haujlwm GUS hauv cov hlwb, cov ntaub so ntswg, thiab tag nrho cov kab mob.

X-Gluc kuj tuaj yeem siv rau hauv kev ntsuas ntau los ntsuas GUS enzyme kev ua haujlwm.Qhov kev siv ntawm cov xim xiav lossis cov khoom tsim tuaj yeem cuam tshuam nrog qib ntawm GUS qhia lossis nws cov kev ua haujlwm enzymatic.

Tsis tas li ntawd, X-Gluc tau ua haujlwm hauv kev tshawb fawb txog noob caj noob ces los kawm txog cov noob caj noob ces, txhawb kev ua haujlwm, thiab cog kev hloov pauv.Nws kuj tau siv rau hauv cov kab mob kab mob rau cloning thiab kuaj GUS fusion proteins.