3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS: 75621-03-3

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) yog ib qho tshuaj ntxuav tes siv hauv biochemistry thiab molecular biology.Nws yog zwitterionic detergent, txhais tau tias nws muaj ob qho tib si zoo thiab tsis zoo them pawg.

CHAPS paub txog nws lub peev xwm los solubilize thiab stabilize membrane proteins, ua rau nws pab tau rau ntau yam kev siv xws li protein extraction, purification, thiab characterization.Nws cuam tshuam lipid-protein kev sib cuam tshuam, tso cai rau cov kua nplaum membrane kom muab rho tawm hauv lawv lub xeev.

Tsis zoo li lwm cov tshuaj detergents, CHAPS kuj yog me me thiab tsis denature feem ntau cov proteins, ua rau nws yog ib qho kev xaiv zoo heev rau kev tswj cov qauv protein thiab kev ua haujlwm thaum sim.Nws tseem tuaj yeem pab tiv thaiv kev sib sau ua ke ntawm cov protein.

CHAPS feem ntau siv rau hauv cov txheej txheem xws li SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric focusing, thiab Western blotting.Nws kuj tau nquag siv hauv cov kev tshawb fawb uas muaj cov enzymes membrane-bound, cov teeb liab hloov, thiab kev sib cuam tshuam ntawm cov protein-lipid.

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Kev siv thiab cuam tshuam

Protein Extraction: CHAPS feem ntau yog siv los rho cov membrane proteins los ntawm cov qauv lom.Nws pab solubilize cov proteins no thiab tswj lawv cov qauv.

Protein Purification: CHAPS yog siv nyob rau hauv ntau yam protein purification cov txheej txheem, xws li affinity chromatography.Nws tuaj yeem muab ntxiv rau purification buffers kom solubilize thiab stabilize membrane proteins thaum lub sij hawm purification txheej txheem.

Protein Characterization: CHAPS feem ntau yog siv rau hauv cov kev tshawb fawb uas cuam tshuam txog cov yam ntxwv ntawm cov proteins membrane.Nws pab tswj cov qauv ntawm cov protein thiab ua haujlwm thaum lub sijhawm sim cov txheej txheem xws li enzyme kev soj ntsuam, kev sib cuam tshuam ntawm cov protein-protein, thiab kev soj ntsuam spectroscopic.

Membrane Protein Studies: Membrane proteins ua lub luag haujlwm tseem ceeb hauv ntau cov txheej txheem ntawm tes.CHAPS feem ntau yog siv rau hauv kev tshawb fawb txog cov teeb liab transduction, ion channel muaj nuj nqi, protein-lipid kev sib cuam tshuam, thiab membrane protein crystallization.

Electrophoresis: CHAPS yog siv rau hauv cov txheej txheem xws li SDS-PAGE thiab isoelectric tsom mus rau solubilize membrane proteins thiab pab txhawb lawv kev sib cais thiab kev tshuaj xyuas.

Cov khoom ntim:

Cov ntaub ntawv ntxiv:

Kev sib xyaw	C32H58N2O7S
Assay	99%
Qhov tshwm sim	Dawb crystalline hmoov
CAS Nr.	75621-03-3
Ntim	Me me thiab bulk
Txee lub neej	2 xyoo
Cia	Khaws rau hauv qhov chaw txias thiab qhuav
Daim ntawv pov thawj	ISO.